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| Registros recuperados: 101 | |
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| Li,Jiang; Hu,Qiushi; Li,Yuquan; Xu,Yuan. |
| An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psychrophilic bacteria; Cold-adapted agarase; Purification; Characterization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300683 |
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| Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina. |
| The production of manganese peroxidase (MnP) from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L) after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Manganese peroxidase; Purification; Characterization. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400012 |
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| Grivaud-le Du, Alicia; Zykwinska, Agata; Sinquin, Corinne; Ratiskol, Jacqueline; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome; Delbarre-ladrat, Christine; Colliec-jouault, Sylvia. |
| Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other... |
| Tipo: Text |
Palavras-chave: Marine Alteromonas infernus; Bacterial exopolysaccharide; Endotoxin; Characterization; Purification; Fermentation; Process. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00397/50818/51601.pdf |
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| Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.. |
| Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337 |
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| Lancellotti,Marcelo; Pace,Fernanda de; Stehling,Eliana Guedes; Villares,Maria Cecília Barisson; Brocchi,Marcelo; Silveira,Wanderley Dias da. |
| Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Haemophilus influenzae; Characterization; Ribotyping; Serotype; Biotype. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500015 |
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| Wang,Haikuan; Zhong,Shaojiong; Ma,Huijing; Zhang,Jie; Qi,Wei. |
| A novel alkaline lipase-producing strain 1-7 identified as Acinetobacter calcoaceticus was isolated from soil samples collected from Bohai Bay, China, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. The lipase from strain 1-7 showed the maximum activity at pH 9.0 under 40ºC. One interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. It is also stable at a broad range of pHs from 4.0 to 10.0 for 24 h. Its catalytic activity was highly enhanced in the presence of Ca2+, Mg2+ and K+, but partially inhibited by Cu2+, Al3+, Fe3+ , Ba2+and Zn2+. The fact that it displays marked stability and activity in the presence of TritonX-100, Tween-20, Tween-80,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline lipase; Acinetobacter calcoaceticus; Characterization; Detergent. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100016 |
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| Neves,Valdir Augusto; Picchi,Douglas Gatte; Silva,Maraiza Aparecida da. |
| Polyphenoloxidase (PPO; EC 1.14.18.1) extracted from Mentha arvensis leaves was isolated by (NH4)2SO4 precipitation and extensive dialysis. Its optimum pH and temperature varied with the substrate. The PPO showed activity with various diphenols. Km values were found 0.825, 0.928 and 7.41mM for caffeic acid, 4-methylcatechol and catechol, respectively. On heat-inactivation, half of the activity was lost after 60 and 15 sec at 70 and 75ºC, respectively. Measuring of residual activity showed a stabilizing effect of sucrose at various temperatures with activation energy (Ea) for inactivation increasing with sucrose concentration from 0 to 40% (w/w). Ea values of 78.13; 80.37; 82.79 and 81.00 kJ/Mol were found for 0, 15; 30 and 40% sucrose, respectively. PPO... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polyphenoloxidase; Mentha arvensis; Characterization; Heat inactivation; Inhibitors. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400025 |
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| Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia. |
| Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared... |
| Tipo: Text |
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf |
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| Otero,Laura; Ducasse,Daniel; Miller,Robert N. G.. |
| Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Olea europaea; Molecular markers; Characterization. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000400015 |
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| Noguez Hernández, Roberto. |
| En la presente investigación, se realizó una caracterización agromorfológica y se identificó taxonómicamente una nueva especie de romerito. Para el cumplimiento de los objetivos, este trabajo se dividió en dos capítulos. En el primero, a partir de caracteres cualitativos y cuantitativos, se caracterizaron y agruparon 70 muestras poblacionales procedentes de plantas cultivadas y silvestres. Los resultados de la investigación a partir de los análisis de correspondencias y discriminante permitieron agrupar la variación del romerito de acuerdo a la zona de colecta en tres grupos. (Centro, Occidente y Norte). Los caracteres ciclo de vida, número de estambres, tipo de crecimiento en etapa vegetativa, días a floración, fructificación y madurez de semilla fueron... |
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Palavras-chave: Romerito; Caracterización; Sueada edulis; Suaeda mexicana; Comestible; Characterization; Edible; Producción de Semillas; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/2107 |
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| Santiago-Luna,Eric G; Carrillo-Rodríguez,José C; Chávez-Servia,José L; Perales-Segovia,Catarino; Heredia-Garcia,Elena. |
| ABSTRACT In a collection of costeño peppers conserved by indigenous producers from the municipalities of Santa Maria Tonameca and Santo Domingo de Morelos, Oaxaca, Mexico, a description and classification of agromorphological variation was undertaken by characterization of 46 populations in a greenhouse distributed under complete randomized block design with three replicates. Days to first flowering, plant and fruit traits and yield per plant were evaluated. Significant differences were detected for all traits except for plant height at 120 days after planting. In a principal component analysis, the variables of green and dry weight of 15 fruits, average fruit weight, number of fruits and yield per plant were the characteristics with major descriptive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capsicum annuum; Phenotypic diversity; Plant genetic resources; Characterization; Multivariate analysis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000300399 |
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| Registros recuperados: 101 | |
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